Composite

Part:BBa_K4800040:Design

Designed by: Chenxi Ma   Group: iGEM23_NJTech-China-B   (2023-10-09)


pTrc99a-YahK


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3627
    Illegal PstI site found at 3639
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3639
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3621
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3627
    Illegal PstI site found at 3639
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3627
    Illegal PstI site found at 3639
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3823
    Illegal BsaI site found at 4891
    Illegal SapI.rc site found at 767


Design Notes

Any restriction sites for EcoRI, XbaI, PstI, SpeI, and NotI have been removed.


Source

None

References